Provided a List has already been mailed to at least once, one can send another Regular Campaign to just those who either DID or DID NOT Open a previous Campaign.
The setting is made on the final Confirmation Page in Campaign Setup, as below.
The fact that a subscriber did or did not Open a Campaign (and if they clicked a link in the Campaign) is automatically recorded for Stats purposes PROVIDED you set “URL Tracking” to Yes in Campaign Setup. One can mail to just those who DID or DID NOT Open as above, based on that data.
However, one cannot create Segments, or Automate moving Subscribers from one List to another based on that data, be it Opens or Clicks. To do that one needs to have Custom Fields set up to record OPENS and CLICKS, and those fields are set up in all new Lists by default.
For both OPENS and CLICKS you can then either “Change” the value in those fields to “Yes” when a Subscriber does open or Click, or set an “Action” that will either “Copy” or “Move” the Subscriber to another List.